| Enigma Software | Ontario |
| Enkidoo AI | |
| ENKON Information Systems, Inc. | British Columbia |
| Enos | |
| EnPoint, Inc. | |
| Enrich Software Corporation | Alberta |
| ENS eLearning Solutions, Inc. | Ontario |
| Ensemble Systems | British Columbia |
| Ensil, Inc. | Ontario |
| EnSoft | Quebec |
| Ensol Systems, Inc. | |
| ENTA Solutions, Inc. | |
| Entasight IT | |
| Entergate, Inc. | Ontario |
| Enterprise Computer Dimension | Quebec |
| Enterprise Managed IT Solutions | Ontario |
| Enterprise Quantum | |
| Enterprise Web Cloud | |
| Entertainment Software Association of Canada | Ontario |
| Enthink, Inc. | Alberta |
| Enticity | Ontario |
| EntirelyDigital | Ontario |
| EntreChefs PME | |
| Entreprendre ici | |
| Entreprendre Sherbrooke | |
| Entrepreneur Roadmap, Inc. | |
| Entrepreneur Web Technologies, Inc. | Quebec |
| Entrepreneuriat Haute-Yamaska | |
| Entrepreneuriat Québec | Quebec |
| Entrepreneurship Society | |
| Entrepreneurship, Practice and Innovation Centre | |
| entrepreneurship@UBC | |
| EntrepreNorth | |
| Entreprise MST, Inc. | |
| Entreprisesavendre.quebec, Inc. | |
| Entrevestor | Nova Scotia |
| Entro | |
| Entry Software Corporation | Ontario |
| Entuitive Workforce, Inc. | |
| EnvirInfo | Quebec |
| Environics Analytics | Ontario |
| Environmental Criminology Research, Inc. | British Columbia |
| Envirosoft | Quebec |
| Envirosoft Corporation | |
| Envisio | British Columbia |
| Envision IT, Inc. | Ontario |
| Envision Online Media, Inc. | Ontario |
| Envision Saint John | |
| envision.ai | Quebec |
| Envoi Technologies, Inc. | |