| Émilien Edmond | Quebec |
| Emily Carr University of Art + Design | British Columbia |
| EMKAL, Inc. | |
| EMM Labs, Inc. | Alberta |
| Emma Services Financiers, Inc. | |
| Emma42, Inc. | |
| Emmatt Digital Solutions, Inc. | Ontario |
| eMonster Solutions | |
| Empathy, Inc. | Ontario |
| Empego Technologies, Inc. | |
| Emperex Corporation | Ontario |
| Emphasize Design, Inc. | |
| Empire Marketing | Alberta |
| Empix Imaging, Inc. | Ontario |
| Emplois Compétences | |
| EmployNXT | |
| Empower Technologies | British Columbia |
| Empower You Web Solutions, Inc. | Ontario |
| Empowered Startups | British Columbia |
| Emprez | Quebec |
| Empty Lot Software, Inc. | Ontario |
| Empyrion Technologies, Inc. | British Columbia |
| EMRlogic | British Columbia |
| emsolutions.ca | Quebec |
| Emspace Marketing | Ontario |
| emtelligent | |
| eMVee Design | |
| EMX Enterprises Ltd | Ontario |
| Emyode | Quebec |
| Emyx Technologies | |
| ENA Electronics, Inc. | |
| EnableUC, Inc. | |
| Enactus Canada | |
| Enaimco | |
| Enara Technologies, Inc. | Ontario |
| Enceladus Consulting, Inc. | British Columbia |
| Encepta | |
| Encircle, Inc. | |
| Encom Wireless | |
| enCompass Solutions Group | British Columbia |
| Encompassing Visions | Alberta |
| Encore Business Solutions | Manitoba |
| Encore Interactive | New Brunswick |
| Encore Market Engagement | |
| ENCQOR | |
| EncrEco | Quebec |
| End to End Networks, Inc. | Ontario |
| ENDA Technologies, Inc. | |
| Endeavor Networks | Ontario |
| Endeavour Solutions, Inc. | Ontario |