| Elli | Quebec |
| Elligno, Inc. | Quebec |
| Ellis Telecom, Inc. | Ontario |
| Ellison Technologies | Ontario |
| Elm Computer Services Ltd | Manitoba |
| Elmo Solutions, Inc. | Quebec |
| Elocity | Ontario |
| Elosoft Online | Ontario |
| eluta.ca | Ontario |
| Elyptic Production, Inc. | Ontario |
| Élyse Giroux | |
| Em Dash Design | Quebec |
| EM Data Consultants | Ontario |
| EMAGIN Clean Technologies, Inc. | |
| Emagine | Ontario |
| EMAGIX, Inc. | |
| Emall | |
| Emarketing-Canada | Quebec |
| eMashup Solutions | Ontario |
| Embedded Access, Inc. | Ontario |
| Ember Metrics | Ontario |
| EMBERS | British Columbia |
| embl.ca | Ontario |
| EMBLÈME Communication | Quebec |
| Embodia, Inc. | |
| Embrace Multi-Graphics | |
| Embraceware Software, Inc. | |
| Embrase | Quebec |
| Embree Communications | Nova Scotia |
| Embrionix | |
| Embvue | Quebec |
| EMC Consulting, Inc. | Ontario |
| EMCI Solutions | |
| EMD Serono Canada | Ontario |
| emd technologies | Quebec |
| Emedia Websites | Manitoba |
| Emeldi Canada Ltd | Ontario |
| Emenki Web Solutions | Ontario |
| Emera ideaHUB | Nova Scotia |
| Emerald Associates | Alberta |
| Emerald City Games | |
| Emerald Presentations | Ontario |
| EmergConnect | |
| Emerge, Inc. | Quebec |
| Emerge2 Digital, Inc. | Ontario |
| Emergence | Prince Edward Island |
| Emergencys | Quebec |
| Emergent Vision Technologies, Inc. | British Columbia |
| EMG Technologies | |
| Emilicious Design | British Columbia |