| Espace-O | |
| Espacenet | Quebec |
| Esphera SynBio | |
| Esplanade Québec | |
| Esplanade Ventures | Quebec |
| Espresso Capital | Ontario |
| Espresso Communication & Design, Inc. | Quebec |
| Esprida Corporation | Ontario |
| ESRI Canada Ltd | Ontario |
| Essence Tech Labs | |
| Essential Data Services | Ontario |
| Essential Designs | British Columbia |
| Essential Multimedia | Ontario |
| Essential Software Solutions | British Columbia |
| Essex Energy Corporation | Ontario |
| Estateably, Inc. | |
| Estatoora | |
| ESTI Consulting Services | Saskatchewan |
| Estimation.ca | Quebec |
| Estimator Corp Canada | Ontario |
| Estrie - Cantons-de-l'Est | |
| Estrie Informatique | |
| Estrinnov | |
| eStruxture Data Centers | Quebec |
| eSubnet Enterprises, Inc. | Ontario |
| eSummum | Quebec |
| ESW IT | Alberta |
| ET Group | |
| eTalent Canada | Ontario |
| Etaliq, Inc. | Ontario |
| ETC Solutions Informatiques, Inc. | |
| ETC. Everything 2 Connect | Ontario |
| eTEL Technologies, Inc. | Ontario |
| Eternal Tech Systems Corporation | |
| Ethereal 3D | Alberta |
| EthicalHosting | Ontario |
| Ethnocare | |
| Ethoca | |
| ETI-NET | Quebec |
| Etico Software, Inc. | Ontario |
| eTimeMachine | Ontario |
| Etineria | Quebec |
| Etios Solutions | |
| ETL Web Solutions | |
| ETO Engineering | |
| Eton College | |
| Eton Systems | Ontario |
| eToolSmith Development | Ontario |
| Etresoft, Inc. | Ontario |
| Etrion Corporation | British Columbia |