| Enders Direct | Ontario |
| EnDesign | |
| Endless Communications | Ontario |
| Endloop Systems, Inc. | Ontario |
| Endoceutics, Inc. | Quebec |
| Endocision | |
| Endoh Collaborative | |
| EndUser, Inc. | |
| EnerCheck Solutions | |
| Enercom Canada, Inc. | Ontario |
| Energetex Engineering | Ontario |
| Energex, Inc. | British Columbia |
| Energhx Green Energy Corporation | Ontario |
| Energia Ventures | New Brunswick |
| Énergie NB Power | New Brunswick |
| Energy NL | |
| Energy Research & Innovation Newfoundland & Labrador | |
| Energy Storage Instruments | Ontario |
| Energy Telecom, Inc. | Ontario |
| EnergyCAP Canada, Inc. | Ontario |
| energyX Solutions, Inc. | |
| Enertec, Inc. | Quebec |
| EnerTech Capital | Ontario |
| Enerteck | |
| Enerza, Inc. | |
| EnEstrie.ca | |
| Enfocom | Alberta |
| EngageFirst Management Consultants | |
| Engel Media | Alberta |
| enGene | |
| enGene Holdings, Inc. | |
| Engenic | British Columbia |
| Engenuity Corporation | Ontario |
| Enghouse Networks | Ontario |
| Enghouse Systems | Ontario |
| Engine 8 Media | Ontario |
| Engine Digital, Inc. | British Columbia |
| Engine X | Ontario |
| Engineered Code Consulting, Inc. | |
| Engineered Multimedia Solutions | Saskatchewan |
| Engineering CPR, Inc. | |
| Engineering Possibilities | |
| Enginehire | |
| Enginess | Ontario |
| Enginuity, Inc. | |
| Engynn Software | Alberta |
| Enhanced Business Systems, Inc. | Alberta |
| Enhansoft | Ontario |
| Enigma Biomedical Group | |
| Enigma Interconnect | British Columbia |