| Envoke | Ontario |
| Envol Strategies | British Columbia |
| Envy Design Co. | |
| Enzo Conception Internet | Quebec |
| Enzuzo | |
| Enzyme Testing Lab | |
| Eocycle | Quebec |
| Eole | Quebec |
| EON Media Corporation | |
| Eos Lightmedia | |
| Eosense, Inc. | |
| ePACT Network Ltd | |
| Eperformance, Inc. | Ontario |
| EPG Computers | |
| Éphémère Creative Ltd | |
| Ephicacy Canada, Inc. | |
| Ephor Technologies, Inc. | Ontario |
| EPI Internet Direct | Ontario |
| Epic | Manitoba |
| Epidote Technology Solutions, Inc. | Ontario |
| Epimatic | Ontario |
| Epineuron Technologies, Inc. | |
| Epiphan Systems, Inc. | Ontario |
| Epitopea Ltd | |
| EPK Training Solutions, Inc. | Ontario |
| ePly Services, Inc. | British Columbia |
| Epodium | |
| EPOK Therapeutics, Inc. | |
| Epro Domains | Quebec |
| Eprom Computer Systems | Ontario |
| EPSI Computer Systems, Inc. | British Columbia |
| Epsidon, Inc. | Ontario |
| Epsilia | |
| Epsilon 5 Engineering | New Brunswick |
| Epsilon Learning Systems | British Columbia |
| Epsilonsys Software, Inc. | Quebec |
| Epson Canada | Ontario |
| Eptix Electronics, Inc. | Quebec |
| EQ Works | Ontario |
| EQ Works | |
| eQOL, Inc. | |
| eQuake Systems | |
| Equasion Business Technologies Consulting, Inc. | Ontario |
| Equator | Ontario |
| eQube | Alberta |
| eQuest | Ontario |
| Equinox Payments | |
| Equinox Software Design Corporation | British Columbia |
| EquipBureau | Quebec |
| Équipe Microfix | Quebec |