| Senet | South Holland |
| Senhive B.V. | |
| Seniorweb Groningen | Groningen |
| Sensara | |
| Sense Health B.V. | |
| Sense-IT | Drenthe |
| SenseGlove | |
| Sensity | |
| Sensius | South Holland |
| Sensor Partners Nederland | North Brabant |
| Sensorfact | |
| Sensoterra B.V. | |
| Sentia | North Holland |
| Sentient Information Systems | North Holland |
| Senwa B.V. | South Holland |
| SEO Advies MKB | |
| SEO Effect | North Holland |
| SEO vrienden | |
| SEO-bureau Optimus Online | |
| Seogi | |
| SEOguru | North Holland |
| SeraNovo B.V. | |
| Serendipity Holding B.V. | |
| Serious VR B.V. | |
| Serrala | North Holland |
| SERVERAREA.COM | North Holland |
| ServerAtSchool | North Holland |
| Serverspace | |
| Servitall B.V. | South Holland |
| SESS Marketing | |
| SET Ventures | North Holland |
| Settly B.V. | |
| Seventury | North Holland |
| Share Council B.V. | |
| SharpMinds | |
| SHIFT | |
| Shift F5 | North Holland |
| Shift Invest | North Holland |
| Shifting Views | North Brabant |
| Shinycore Software | Friesland |
| ShipSmarter.com B.V. | |
| Shopnet | North Holland |
| Shoppagina B.V. | Utrecht |
| ShowMedia | Overijssel |
| Shuberg Philis | North Holland |
| Shypple | South Holland |
| SIBCO B.V. | North Brabant |
| Sibero ICT | North Brabant |
| Sibö B.V. | |
| Sibren Strategy & Design | North Holland |