| Solid Hosting | Overijssel |
| Solid Partners | Overijssel |
| Solid Ventures | |
| Solidflux | North Holland |
| Soliton Systems Europe N.V. | North Holland |
| Solnet Group | |
| Solomax Internet B.V. | |
| Soluso | Gelderland |
| Solutions Design | South Holland |
| Solutions Factory | |
| Solvec Internet Solutions | South Holland |
| Solventa | Utrecht |
| Solviteers Cloud & Infradiensten | |
| Solynta | Gelderland |
| Sonantic | South Holland |
| SOS Solutions | |
| Source.ag | |
| Source.ag B.V. | |
| Sourceful ICT | |
| Sourcelab | |
| Sourcia | |
| Sovon | |
| SOWNet Technologies B.V. | |
| Spaaza Technologies B.V. | |
| Space Business Innovation Centre Noordwijk | |
| Space Promotion B.V. | Limburg |
| Spaces Holding B.V. | |
| Spaceti | North Holland |
| Spare IT | North Brabant |
| SparkNano | North Brabant |
| Spartner | |
| Speak B.V. | Friesland |
| Specialist Retail Systems B.V. | Flevoland |
| SpeechCloud | |
| Spel-Maker | North Holland |
| Spherical | |
| Spice Productions | Limburg |
| SPIE Nederland B.V. | |
| Spil Games | North Holland |
| Spinoza Centre for Neuroimaging | |
| Spinware Solutions B.V. | South Holland |
| Spirit IT B.V. | North Brabant |
| Spirit Solutions B.V. | Limburg |
| Spitters | North Brabant |
| Sportmonks | Overijssel |
| Spot Software B.V. | North Holland |
| Spotler | South Holland |
| Spotr | |
| Spotzer | North Holland |
| Spraakmaker Telecom | Gelderland |