| SaaSplaza International | North Holland |
| Sab Training B.V. | South Holland |
| SabanWorld | South Holland |
| Sabiad | |
| Sabra Systems B.V. | |
| Saffron | |
| Sageon B.V. | |
| Saia Agrobotics B.V. | |
| SailRouter | South Holland |
| Sairopa B.V. | |
| SALAR Software | North Brabant |
| Salarise | |
| Sales Rocks | South Holland |
| SalesManager Software | North Holland |
| Salland Engineering (Europe) B.V. | Overijssel |
| Salonized B.V. | |
| Saltrex | |
| Salvia BioElectronics | |
| SamMobile B.V. | |
| Samotics | South Holland |
| Samskip | |
| Sanaga Ventures | North Holland |
| Sanaga Ventures | |
| SanBio B.V. | |
| Sandra Kingma Grafische Vormgeving | Gelderland |
| Sanicom Telecommunicatie B.V. | South Holland |
| Sanquin | |
| Santhos | Drenthe |
| Sapenta | South Holland |
| Sapreme Technologies | Utrecht |
| Sark7 | South Holland |
| Satellietdiscount.com | Limburg |
| Satelligence | Utrecht |
| Satsearch B.V. | |
| Satys B.V. | |
| Savana B.V. | |
| Saxion University of Applied Sciences | Overijssel |
| Saylus | Gelderland |
| SBeffect | Zeeland |
| Scala Solutions | |
| Scaldis ICT B.V. | |
| Scale Cities Alliance | North Holland |
| ScaleX Ventures | North Holland |
| Scamander | Utrecht |
| ScanmarQED | Utrecht |
| Scenic Biotech | North Holland |
| SchedJoules | |
| SchiozziWeb | |
| Schiphol Telematics B.V. | North Holland |
| Schoevers | |