| Science Park Amsterdam | North Holland |
| Scientific Volume Imaging | North Holland |
| Scinvivo | |
| SciSports | |
| Scitodate B.V. | |
| Scoopexpert B.V. | |
| Scope Biosciences B.V. | |
| SCOPE marketing technology B.V. | North Holland |
| Scopic B.V. | North Holland |
| Score Agency | |
| SCOS | North Holland |
| ScraperBox | North Holland |
| ScreenCheck B.V. | South Holland |
| ScreenPoint Medical B.V. | |
| SDB Groep | |
| SDB Java | South Holland |
| SDC B.V. | Gelderland |
| SDIM | North Holland |
| SDO Hogeschool | |
| Sdu | |
| Seal It Services B.V. | South Holland |
| Search Signals | |
| Searchflow | |
| SearchSoftware | |
| Seceurtronics | North Holland |
| SECOMP Nederland GmbH | South Holland |
| SecuData | North Brabant |
| SecuPlus B.V. | Zeeland |
| Secure Internet Machines (SIM) B.V. | North Holland |
| Secure Internet Services | South Holland |
| SecureConsult | |
| Secured by Design | |
| Secureness B.V. | North Holland |
| Security Academy | |
| Security Delta | |
| SecurityHive | |
| Secusystems | |
| SeedPact | |
| Seenons | |
| Sekurno | |
| Self-screen B.V. | |
| Selles Automatisering B.V. | Overijssel |
| Semantica | South Holland |
| SemLab | South Holland |
| Semmie | |
| Sempro | |
| Sencio B.V. | |
| Send AI | |
| SendCloud | North Brabant |
| Seneca | South Holland |