| Re-Order-It | Utrecht |
| Re:ason Digital Empowerment | South Holland |
| Reach Digital | |
| React Summit | North Holland |
| RealHosting | North Holland |
| RealSolution | |
| Realtime Register B.V. | Overijssel |
| Realworld Systems B.V. | Gelderland |
| Recall | |
| ReCheck B.V. | Limburg |
| Recommendme | |
| Recrout | North Holland |
| Recruitee.com | North Holland |
| RecruitmentTechnologies | North Holland |
| Red Data | |
| Red Kubes B.V. | |
| Red Star Electronics B.V. | South Holland |
| Red to Blue | |
| Redakt B.V. | |
| Redevco B.V. | |
| Redhound Internetbureau | Overijssel |
| Redkiwi | South Holland |
| RedMedTech Discovery Fund | Gelderland |
| RedVan Solution B.V. | |
| Reflecta Automation B.V. | South Holland |
| Reflex Online | Gelderland |
| Refreshworks B.V. | |
| Registratie.nl | Gelderland |
| Reinier Martin | |
| RekenTest | South Holland |
| Relay42 | North Holland |
| Relive B.V. | |
| Relive B.V. | |
| ReloadSEO B.V. | |
| Relu B.V. | |
| Rely Websolutions | North Holland |
| Relyon Holding B.V. | South Holland |
| ReMedia | Groningen |
| Remedy IT | Gelderland |
| Remind-IT | Limburg |
| Remy | |
| RENA Electronica B.V. | North Brabant |
| Renaissance IT | South Holland |
| Renoon | North Holland |
| Reprise Digital | |
| Repsy | |
| Research Manager | Overijssel |
| Researchschool CaRe | |
| Resello | Overijssel |
| Resleeve | |