| SecondNature | Tel Aviv District |
| SecureTeam Software Ltd | Central District |
| See Security College | Tel Aviv District |
| Seebo Interactive Ltd | Tel Aviv District |
| SeedIL Ventures | Tel Aviv District |
| SeeMetrics | |
| Seenet | Tel Aviv District |
| Segal Software & Computers | Tel Aviv District |
| Segment | Central District |
| Seguro | Central District |
| SELA Ltd | Northern District |
| SEM2U Digital Marketing | Jerusalem District |
| Senso-Optics | Northern District |
| Sentra | Tel Aviv District |
| SEO Creative Ltd | Central District |
| SEO Israel | |
| SEO Jerusalem | |
| SEO Jobs Finder | Tel Aviv District |
| SEO Simple Ltd | Tel Aviv District |
| SEOIsrael | Tel Aviv District |
| Seperia | |
| Septier Communciation Ltd | Central District |
| Sequentify Ltd | Central District |
| Seraphic Ltd | |
| Sergata | Tel Aviv District |
| Serv-IL | Tel Aviv District |
| ServicesApp | Tel Aviv District |
| SerVision Ltd | Tel Aviv District |
| Servotronix Motion Control Ltd | Central District |
| Setpoint | Tel Aviv District |
| Shafir Production Systems Ltd | |
| Shaked Ventures | Tel Aviv District |
| Shalem College | |
| ShareRoute Ltd | Tel Aviv District |
| Sharkor | Tel Aviv District |
| She Codes | Tel Aviv District |
| Sheleg Software | Tel Aviv District |
| Shenkar College of Engineering, Design and Art | Tel Aviv District |
| Shield | Tel Aviv District |
| Shina Systems Ltd | Haifa District |
| Shine | |
| Shkedim Studio | Tel Aviv District |
| SHL Telemedecine | Tel Aviv District |
| Shm.co.il | Tel Aviv District |
| ShopCenter | Tel Aviv District |
| Shopic | Tel Aviv District |
| ShopSystem | Tel Aviv District |
| Shva | |
| Siftech | Jerusalem District |
| Sig Software | Tel Aviv District |