| Softweave | Central District |
| SoftXML | Tel Aviv District |
| Sogo Digital UI & UX Development | Central District |
| Sol-Gel Technologies Ltd | Central District |
| Solan Technologies Ltd | Haifa District |
| SolarEdge Technologies, Inc. | Tel Aviv District |
| SolCold | Central District |
| SolidRun Ltd | Northern District |
| Solidus | Tel Aviv District |
| Solvo | |
| SONGO Media | Tel Aviv District |
| Sonovia Tech | |
| Sosa | Tel Aviv District |
| Source Defense | Southern District |
| SouthUp | Southern District |
| SpaceIL | Tel Aviv District |
| Spartans Technologies | Tel Aviv District |
| SPD Hosting Ltd | |
| SPD Web Hosting | Tel Aviv District |
| Spectral Cyber Technologies Ltd | Tel Aviv District |
| Speedata | Central District |
| Speeds | Tel Aviv District |
| Spera Security | Tel Aviv District |
| Spincare | |
| Spinner Design Studio | Central District |
| Spiral Solutions Interactive Marketing | |
| Spiralic | |
| SpliSense | Jerusalem District |
| SpotNet | |
| Spring Games | Tel Aviv District |
| Spring Ventures | Tel Aviv District |
| SPS Smart Printing Solutions Ltd | Central District |
| SQLink Group | Tel Aviv District |
| SRV Internet Services | Tel Aviv District |
| StageOne Ventures | Tel Aviv District |
| Stands Ltd | Tel Aviv District |
| Star Ventures | Tel Aviv District |
| StarkWare Industries | Central District |
| Start Israel | Tel Aviv District |
| Start-Up Nation Central | Tel Aviv District |
| Start-Up Nation Finder | Tel Aviv District |
| Startup Camel | Tel Aviv District |
| Startup Stash | Tel Aviv District |
| StartupHub.ai | Tel Aviv District |
| State Of Mind Ventures | Tel Aviv District |
| SteelKiwi, Inc. | Tel Aviv District |
| Step Of Mind Ltd | Tel Aviv District |
| Sternum | Tel Aviv District |
| StoreDot | |
| StoreYa Feed Ltd | |