| North First Ventures | Tel Aviv District |
| Nostromo Energy | Central District |
| Notebook Club | Tel Aviv District |
| NoTraffic | Tel Aviv District |
| Nova Measuring Instruments Ltd | Central District |
| Novadea, Inc. | Tel Aviv District |
| Novamed Ltd | |
| Novawind | Tel Aviv District |
| Novel Power Systems Ltd | |
| Novidea | |
| Novilis | Tel Aviv District |
| NoviSign | |
| Novu | Tel Aviv District |
| NSLComm | |
| nSoft | |
| nSure.ai Ltd | Tel Aviv District |
| NTT | Tel Aviv District |
| Nura Global Innovation Lab | |
| Nurami Medical | Haifa District |
| NurExone Biologic | Haifa District |
| Nutrino Health Ltd | Tel Aviv District |
| NYX Technologies | Haifa District |
| O.G. Tech Ventures | Tel Aviv District |
| O.R. Technologies Ltd | Tel Aviv District |
| OAK Computer Systems Ltd | Central District |
| OCON Therapeutics | |
| Octopai | Central District |
| OcuCloud | Tel Aviv District |
| Odix | Central District |
| Ofek Dist | |
| OfficeTrack | Central District |
| OFIL Systems | |
| Oktopost Technologies | Tel Aviv District |
| Oligo | Tel Aviv District |
| Olive Diagnostics | Jerusalem District |
| Olive Tree Venture | |
| Olmait | |
| Olsale | Tel Aviv District |
| OM2 | Tel Aviv District |
| OmegaIsrael | Tel Aviv District |
| Omnis Digital Agency Ltd | |
| Omnisol Information Systems Ltd | Haifa District |
| Omnix Medical Ltd | |
| On Track Innovations Ltd | Northern District |
| One Technologies | |
| Onebeat Ltd | |
| Ongage | Tel Aviv District |
| Ono Academic College | |
| OOONA.NET Ltd | Tel Aviv District |
| Oosto | Tel Aviv District |