| Open University of Israel | Central District |
| OpenApp | Jerusalem District |
| OpenMind Software Development Ltd | Tel Aviv District |
| OpenWeb Ninja | |
| OPGAL | |
| Ophir Optronics Solutions Ltd | |
| Opinion State Ltd | |
| Opisoft | Tel Aviv District |
| OPKO Biologis Ltd | Southern District |
| OpteamX Ltd | Tel Aviv District |
| Optibus | Tel Aviv District |
| Optical Imaging Ltd | Central District |
| Optical Metrology Ltd | Jerusalem District |
| Optimal Plus Ltd | Tel Aviv District |
| Optimization Solutions | Jerusalem District |
| Optimove | Tel Aviv District |
| Optimus | |
| Optoacoustics Ltd | |
| Optronics Engineering | |
| Oracle Israel | Central District |
| Oranim Academic College of Education | |
| Orbit Communication Systems | Central District |
| Orbit Communications Systems Ltd | |
| Orbium Software | Central District |
| Orbotech Ltd | Central District |
| ORCA | |
| OrCam | Jerusalem District |
| Orchid | |
| Oriental Computer Systems | Tel Aviv District |
| Oriient New Media Ltd | Tel Aviv District |
| Orpak Systems Ltd | Tel Aviv District |
| Orpheus Medical Ltd | Haifa District |
| OrSense Ltd | Tel Aviv District |
| Orsyx | Central District |
| Ort Braude College of Engineering | Northern District |
| ORT Israel, Technology & Science Educational Network | |
| ORTRA Ltd | |
| Oryzn Capital | Tel Aviv District |
| Oscar Sami Electronics Ltd | |
| Other Sources Energy Group Ltd | Tel Aviv District |
| Otipo | Tel Aviv District |
| Otoma | Tel Aviv District |
| Otonomo | Tel Aviv District |
| OTOT Electronics Ltd | Central District |
| Ottopia Technologies Ltd | Tel Aviv District |
| OurCrowd Management Ltd | Jerusalem District |
| Ovalsec | |
| Over-Sat | |
| Overcut | |
| Overwolf | Tel Aviv District |