| StudentSphere | |
| Studeo | |
| Studhause Technologies | |
| Studio 13 | Ontario |
| Studio 63 | |
| Studio 82 | Ontario |
| Studio Altkey, Inc. | Quebec |
| Studio Anto Design, Inc. | Quebec |
| Studio Beau | |
| Studio Braun | Ontario |
| Studio Chammo | |
| Studio créatif Coloc | Quebec |
| Studio Function, Inc. | Ontario |
| Studio Gibbous | Ontario |
| Studio Grafik | Quebec |
| Studio Locale | |
| Studio Media Web | Quebec |
| Studio Rose Flash | |
| Studio RuniQ | |
| Studio Web Ozone | Quebec |
| Studio Webloft, Inc. | Quebec |
| Studio XP | Quebec |
| Studio ZV | |
| Studiochartreuse | |
| Studiothink | British Columbia |
| Study4As | |
| Studyo | |
| StyleLabs | |
| StyleLabs Marketing Group, Inc. | Alberta |
| Stylex Media Design | Ontario |
| StylinDESIGn | Ontario |
| Stylla-Web | |
| Stymiest & Associates | |
| Sub-Quantum Businesses | |
| SubC Imaging | |
| Subgraph | Quebec |
| Sublink | New Brunswick |
| Submission Monster | |
| Submit AI Tools | |
| SUBNET Solutions, Inc. | Alberta |
| Subséquent, Inc. | |
| SubSpace Communications, Inc. | Ontario |
| Subtitle Designs | Quebec |
| Succeed Sooner Consulting, Inc. | Ontario |
| Success Impact | Ontario |
| SucSeed | |
| Sudden Technologies | British Columbia |
| Sue Sutcliffe | Ontario |
| Sugar Coded Designs | British Columbia |
| Sugeri, Inc. | Ontario |