| Straight-Up Digital Marketing | |
| Strainprint Technologies Ltd | |
| Strand360 | |
| Strata Health Solutions, Inc. | Alberta |
| Strata Research Ltd | |
| Stratabase | British Columbia |
| StrataView | Ontario |
| Stratège Média | Quebec |
| Strategic Analysis | Ontario |
| Strategic Management Force, Inc. | |
| Strategic Technology Associates | New Brunswick |
| Stratégie AgiLean, Inc. | Quebec |
| Strategis Consulting Group, Inc. | British Columbia |
| Strategy Cube | Ontario |
| Strategy Institute | Ontario |
| Stratéjia | |
| Strateolab | |
| Stratford Group Ltd | |
| Strathcom Media | Alberta |
| Strathcona Solutions, Inc. | |
| StratLab Marketing | |
| Strato Automation | |
| StratumAI | |
| Stratwell Strategic Solutions, Inc. | Ontario |
| Stratwit Solutions Ltd | |
| Strawhouse, Inc. | |
| Stream Data Systems | Alberta |
| StreamingFast | |
| Streamline | Ontario |
| Streamroll Technologies, Inc. | |
| StreamScan | Quebec |
| Streamwave Communications Corporation | Ontario |
| Streamworks Tech | |
| Street Context | |
| Street Contxt | Ontario |
| StreetPerfect | Ontario |
| Stride Graphics & Web Design | |
| Strider, Inc. | Ontario |
| Striving Styles | |
| StrobelTEK | |
| Stromcore Energy, Inc. | |
| Strong Data Automation | |
| Strongpoint | |
| StrucSoft Solutions | Quebec |
| Structura Biotechnology, Inc. | |
| Strut Creative | |
| Strutta | |
| Stryve | Ontario |
| Stubbs Communications, Inc. | Ontario |
| Students for the Exploration and Development of Space Canada | |