| Stewart Blusson Quantum Matter Institute | British Columbia |
| Stewart Specialty Risk Underwriting Ltd | |
| STI Informatique | Quebec |
| STI Service Télécommunications | Quebec |
| STI Technologies Ltd | |
| Stich Media | Ontario |
| Sticky Brain Studios, Inc. | Ontario |
| StickyPages | |
| Stikky Media | British Columbia |
| Stillwater Capital Corporation | |
| Stinger Games | British Columbia |
| STIQ | |
| Stir Marketing | British Columbia |
| STJ Retail Corporation | Ontario |
| STLM, Inc. | Quebec |
| Stockholm Syndrome.AI | Quebec |
| Stocksy | |
| Stone Steps, Inc. | Ontario |
| Stonefield Software, Inc. | Saskatchewan |
| Stonefield Systems Group, Inc. | Saskatchewan |
| StonePaper | |
| StoneShare | Quebec |
| Stonewood Group, Inc. | |
| Stoneworks Technologies, Inc. | Ontario |
| Storage Clarity | Alberta |
| Storage Guardian, Inc. | Ontario |
| Storage-Backup-Archive.com | Ontario |
| Storageflex | Ontario |
| Storagepipe Solutions, Inc. | Ontario |
| StorageQuest | Ontario |
| Store4U Business Solutions | |
| Storeo, Inc. | |
| Stork Supply | |
| StorLogic | Nova Scotia |
| Storm Applied Technologies | |
| Storm Internet Services | Ontario |
| Stormboard | |
| StormCloud | |
| StormWeb Canada Hosting, Inc. | Ontario |
| Stormy Weather Software | Ontario |
| Storyboard Solutions, Inc. | Ontario |
| StoryTap Technologies, Inc. | |
| Storytime Capital | |
| STR Automatisation | |
| STR Micro | Quebec |
| STR Solutions point de vente | Quebec |
| STR-SpeechTech Ltd | British Columbia |
| Stra Software | Quebec |
| Stradigi AI | Quebec |
| Straight Fire | |