| eDNAtec | |
| Edovia, Inc. | Quebec |
| EDP Live | |
| eDreamWeb | Quebec |
| EDT Computers | British Columbia |
| Edu Performance | Quebec |
| EduCode Academy | |
| Edumicro | Quebec |
| EduSoftMax | Ontario |
| Edutechno | |
| Edvance Software, Inc. | Quebec |
| Edvisor | |
| Eeckweb design | |
| Eeyou Communications Network | Quebec |
| Effective Contact Management | Ontario |
| Effecto | Quebec |
| Effenti, Inc. | Quebec |
| Effet Cumulatif | |
| Effet Web | |
| Effgis | Quebec |
| Efficiate | |
| Efficiency Nova Scotia | |
| EfficiencyOne | |
| Efficiens, Inc. | Quebec |
| Efficient Building Automation Corporation | British Columbia |
| Eflyermaker | Quebec |
| EFNet #Winprog | British Columbia |
| EFOQUS Canada | |
| EFT Canada, Inc. | Ontario |
| eFusion Technologies, Inc. | British Columbia |
| Egami Creative Agency | |
| EGATE Networks | Ontario |
| Eggbeater Creative, Inc. | British Columbia |
| Eggcitables | Nova Scotia |
| Eggplant Studios | British Columbia |
| Eggs Media | |
| EGP Technovirtuel, Inc. | Quebec |
| Egriculture | Ontario |
| Eguana Technologies | Alberta |
| eGuest | Alberta |
| Egwald Web Services | |
| Ehco-Matic, Inc. | Quebec |
| eHealth Ontario | Ontario |
| ehMac.ca | Ontario |
| EHS Analytics | |
| ehsAI | |
| EHVA, Inc. | Quebec |
| EI Catalyst, Inc. | Ontario |
| EiconDev | Quebec |
| EICsoft | Ontario |