| SAWebhost.Com | Gauteng |
| SCA - Carolin Botha | Western Cape |
| Scott Web Designs Studio | KwaZulu-Natal |
| Screenquip | Gauteng |
| ScreenVision SA | Gauteng |
| SCS Space (Pty) Ltd | |
| Seacom | Western Cape |
| Searchmedia | |
| SecuPlus | KwaZulu-Natal |
| Secure Time Technology | Gauteng |
| Sedibeng TVET College | |
| Seed Academy | Gauteng |
| Sefako Makgatho Health Sciences University | |
| Seidor Networks | Gauteng |
| Sekhukhune TVET College | |
| Semantica Digital (Pty) Ltd | Western Cape |
| Sentech | Gauteng |
| SEO Specialist | |
| SEO Studio | Western Cape |
| SEOStrategicSolutions.com | Gauteng |
| Seven Lemons Design Studio CC | Free State |
| SevenC | Gauteng |
| Shalesh Design Consultants (Pty) Ltd | Western Cape |
| Shapeshift | Western Cape |
| Sherpa | Western Cape |
| ShipShape Software | Gauteng |
| Shopkeeper Solutions CC | Eastern Cape |
| Shopstar | Western Cape |
| SignalRSA | Gauteng |
| Silicon Cape Initiative | Western Cape |
| Silicon Overdrive | Western Cape |
| Silver Linings Technologies | |
| SilverSky | Western Cape |
| Silversoft (Pty) Ltd | Gauteng |
| Silvertubes | Western Cape |
| Simodisa Start-Up | Gauteng |
| SimpliFind | |
| Simply Graphic | |
| Simulation Engineering Technologies (Pty) Ltd | Gauteng |
| Sinetech (Pty) Ltd | Gauteng |
| SITA | Gauteng |
| SiteCreations SA | Gauteng |
| SiteMeUp Online Marketing (Pty) Ltd | Western Cape |
| SitesDeveloper | |
| SiteWorx IT Services | Western Cape |
| SIVOXI | |
| Six Degrees | |
| Six Digital | Gauteng |
| Six Sigma South Africa | Western Cape |
| Siyelo | Western Cape |