| SpotlightGB | |
| Spoton Software Training | Lincolnshire |
| Spotted Zebra | |
| Spottitt Ltd | |
| Spotty Dog Digital Marketing | |
| Spotty Media Ltd | |
| Sprie | |
| Springer Marketing Services Ltd | Cornwall |
| Springpod | |
| Springwise | |
| Springwood Web Solutions | Merseyside |
| SPRINT - SPace Research and Innovation Network for Technology | |
| Sprint Computer Repairs Ltd | West Lothian |
| Sprint Innovations Ltd | |
| Sprintbase | |
| Sprive | |
| Sprout.ai | Greater London |
| SPT Labtech Ltd | |
| SPTS Technologies Ltd | |
| Spudd Mobile Ltd | |
| Spur Electron Ltd | |
| Spur Therapeutics | |
| Spurwing Agency Ltd | |
| Spusu UK Ltd | Greater London |
| Sputnik Digital | Greater Manchester |
| SpyBiotech | Oxfordshire |
| SpyBioTech Ltd | |
| SpyreSync | |
| Spyro IT Solutions | |
| SQA Consulting Ltd | |
| SQL Help | |
| SQLFluff Ltd | |
| SQLServerCentral | Cambridgeshire |
| SqrOne Ltd | |
| Square 1 Communications Ltd | Wiltshire |
| Square One | Gloucestershire |
| Square One | |
| Square Root Solutions UK | |
| Square Systems Ltd | Bristol |
| Squarebird Agency Ltd | |
| Squashed Pixel Ltd | |
| Squee | |
| Squibble Ltd | |
| Squint / Opera | |
| Squircle | |
| Squire Technologies Ltd | Dorset |
| Srglopix | |
| SRO Solutions Ltd | Greater Manchester |
| Srotas Health | |
| SRT Marine Systems plc | |