| Profi Credit Czech, a.s. | |
| Profinet, s.r.o. | |
| Profinit EU, s.r.o. | Prague |
| Profitux.cz | Liberec |
| ProfiWH | Olomouc |
| Proidea.digital | Ústí nad Labem |
| Prointernet, s.r.o. | South Bohemian |
| ProjectSoft HK, a.s. | |
| PROKOP software, s.r.o. | Prague |
| PromethistAI, a.s. | |
| Promotic | Moravian-Silesian |
| Prospeks-IT.cz | |
| Protean, s.r.o. | |
| Protronix, s.r.o. | Pardubice |
| Prusa Research, a.s. | |
| Prusa Research, a.s. | |
| PS Media s.r.o. | Moravian-Silesian |
| PUX, s.r.o. | South Moravian |
| PyCon CZ | Moravian-Silesian |
| PZK Brno, a.s. | |
| Q-net CZ | South Moravian |
| Q2 Interactive, s.r.o. | South Moravian |
| QCM | South Moravian |
| Qore Technologies, s.r.o. | Prague |
| Quant Retial, s.r.o. | Central Bohemian |
| Quantasoft | Prague |
| Quantcom | |
| Quantum Phi, s.r.o. | |
| Qubit Conference | Prague |
| Queen's Software | Olomouc |
| Query Digital, s.r.o. | Prague |
| Quinta-Analytica, s.r.o. | |
| Qusion | |
| Racom, s.r.o. | Prague |
| Radek Chalupa | Prague |
| Raiffeisen stavební spořitelna, a.s. | |
| Randstad HR Solutions, s.r.o. | |
| Range Software | Central Bohemian |
| Ranketta, s.r.o. | |
| RaRe Technologies | South Moravian |
| Rascasone, s.r.o. | |
| RAYNET, s.r.o. | Moravian-Silesian |
| RAZDVA, s.r.o. | |
| Realpad | |
| Realpad Software Ltd | Prague |
| RealWorld Graphics | Ústí nad Labem |
| REBEX ČR, s.r.o. | Prague |
| RechbergNet, s.r.o. | Moravian-Silesian |
| Redamp Security, s.r.o. | |
| Reflex Capital SE | |