| ReFox | Pardubice |
| Registrace-domény.cz | Prague |
| RegulTech Servis, s.r.o. | |
| regZone.cz | South Moravian |
| Rekenber | |
| Religis, s.r.o. | Moravian-Silesian |
| REMONT Software | Prague |
| RemoteFlight | |
| Resco, s.r.o. | Prague |
| Resistant.AI | Prague |
| RESPECT, a.s. | |
| Revolgy Business Solutions, a.s. | |
| Revolt BI, s.r.o. | |
| Rezolv Energy | |
| RIGANTI, s.r.o. | |
| Ringil, s.r.o. | |
| RiteTag | |
| Robin Holesinsky | |
| Robotech SW, a.s. | |
| Robotict | |
| ROBOTSYSTEM, s.r.o. | |
| RoboTwin | |
| Rockaway Capital | |
| Rockaway X | |
| Rockero | |
| Rockstar Software International | |
| Roger, a.s. | |
| Rohlik Group, a.s. | |
| Rohlik.cz | |
| ROI Hunter | |
| Roivenue, s.r.o. | Prague |
| Root.cz | Prague |
| ROPAK v.o.s. | Plzeň |
| ROT-HSware, spol. s r.o. | |
| Roubec Software | South Moravian |
| Ryston Electronics, s.r.o. | Prague |
| S&G Consulting, s.r.o. | |
| S-Case, s.r.o. | |
| S-Comp | Prague |
| S.I.C., spol. s r.o. | Prague |
| S9Y, s.r.o. | |
| Sabiostar | Prague |
| Sabre, s.r.o. | Prague |
| SaferPass, s.r.o. | |
| Safetica Technologies, s.r.o. | Prague |
| Salsita | |
| Salsita, s.r.o. | Prague |
| SALTO spol. s r.o. | South Moravian |
| SALTO, spol. s r.o. | |
| Salu.cz | Prague |