| Nativa Enterprise, s.r.o. | Moravian-Silesian |
| Navigo3 | South Moravian |
| Navisat, s.r.o. | Prague |
| Navisys, s.r.o. | |
| NECOSS, s.r.o. | South Bohemian |
| NejŘemeslníci.cz | |
| Nelisa, s.r.o. | |
| Neofema | Hradec Králové |
| Neon Zlín, s.r.o. | |
| Neronian Studios | |
| Net Magnet, s.r.o. | |
| NET Partners, a.s. | |
| NET service solution, s.r.o. | |
| Net-System, s.r.o. | |
| NetDirect | Moravian-Silesian |
| Neternity Group, spol. s r.o. | Prague |
| Netfox, s.r.o. | |
| Netglade | |
| Nethost, s.r.o. | Prague |
| Netia, s.r.o. | |
| NetIng, spol. s r.o. | |
| NetRex, s.r.o. | |
| NETservis, s.r.o. | Prague |
| Netshark.cz | |
| NETTO Electronics, s.r.o. | |
| NETVOR | |
| NetWings, s.r.o. | South Moravian |
| NeuronSW SE | |
| Neutral czFree eXchange | Central Bohemian |
| Newton University Group, s.r.o. | |
| NEWTON University Group, s.r.o. | |
| Nexnet, a.s. | |
| Next Digital | |
| Next Vision | |
| Nextap Solutions, s.r.o. | |
| Nextnative | |
| NextSoft, spol. s r.o. | |
| Nexum Trilog, a.s. | Prague |
| NICOM | South Moravian |
| Nikitta | South Moravian |
| NIX.CZ, z.s.p.o. | Prague |
| Node5 Praha | |
| Noise Amplifier Studio | |
| Nordic Telecom | |
| Notebook.cz | Prague |
| NOTIA Software | Prague |
| Notum | |
| Novasoft, spol. s r.o. | |
| NQhost | Prague |
| NSW, s.r.o. | Prague |