| Škoda Auto DigiLab, s.r.o. | |
| Škoda Auto Vysoká škola | |
| SKOUMAL, s.r.o. | |
| SkyFox Labs, s.r.o. | |
| SkyMaps | |
| Skyvia | |
| Slezská univerzita v Opavě | |
| Slezská univerzita v Opavě - Matematický ústav v Opavě | Moravian-Silesian |
| Smal Software Development, s.r.o. | |
| Smart Heating Technology, s.r.o. | |
| SMART Office & Companies, s.r.o. | |
| Smart Prague | Prague |
| Smart Technologies, s.r.o. | |
| Smart4Web | |
| Smartdata, s.r.o. | Prague |
| SmartEmailing | |
| SmartGIS, s.r.o. | Olomouc |
| SmartGuide | |
| SMARTLINK, s.r.o. | South Bohemian |
| Smartlook | |
| Smartsupp | |
| SMSmidlet | Prague |
| SNADNEE | |
| Snapstack | |
| SnowlyCode, s.r.o. | |
| Socialbakers, a.s. | Prague |
| Socialsharks | |
| SofCon, spol. s r.o. | Prague |
| SOFTbit software, s.r.o. | |
| SOFTconsult, spol. s r.o. | |
| Softec CZ, spol. s r.o. | |
| SoftEU, s.r.o. | Plzeň |
| SoftHouse, s.r.o. | Plzeň |
| Softmarket, spol. s r.o. | Prague |
| Softopus, spol. s r.o. | |
| Software Development Europe, s.r.o. | South Moravian |
| Software602, a.s. | Prague |
| Sokrates systems, s.r.o. | |
| Solarmont, s.r.o. | |
| SolarPartner, s.r.o. | |
| Solcode, s.r.o. | |
| SoliCAD, s.r.o. | Central Bohemian |
| SolidVision | South Moravian |
| Solution | |
| Some move, s.r.o. | |
| SONET, spol. s r.o. | |
| SoneTech, s.r.o. | |
| SOTIO Biotech a.s. | Prague |
| Sotio Biotech, a.s. | |
| Soulmates Ventures | |