| Sprague Europe B.V. | Gelderland |
| Sprigs | North Holland |
| SpringTeam B.V. | Utrecht |
| SpringTree | |
| Sprinque B.V. | |
| SPRINT Robotics | |
| spriteCloud B.V. | |
| Spruit Digital | |
| Spryng B.V. | North Holland |
| SPS Holding B.V. | South Holland |
| SPS International | Gelderland |
| SQR.nl | North Holland |
| Squads B.V. | |
| Square One Solutions B.V. | North Holland |
| SquareDesign | Gelderland |
| Squeezely | |
| SRON | Utrecht |
| SSP Simple Soft Products | Flevoland |
| Stackscale B.V. | |
| StackState | |
| Staffyou | |
| Stallion Automatisering B.V. | North Brabant |
| StanDev | |
| Starodub B.V. | |
| Starred | North Holland |
| Start People | |
| StartDock | |
| Starteenwinkel | |
| StartGreen Capital | |
| StartHub Wageningen | Gelderland |
| StartLife | Gelderland |
| Startlife | |
| Startpage | South Holland |
| Startpunt Internetn | Gelderland |
| Startup Almere | |
| Startup Amsterdam | North Holland |
| Startup Foundation | |
| Startup in Residence | North Holland |
| StartUp Scan | North Holland |
| Startup Twente | Overijssel |
| Startup Village | |
| Startupboot | |
| Startupbootcamp Bold Action | North Holland |
| Startupbootcamp Sustainability | North Holland |
| StartupTalks | |
| Stat.me B.V. | |
| Staten Biotechnology B.V. | |
| Steam Data Suite | North Holland |
| Stebis B.V. | Gelderland |
| Steens & Partners | |