| Quantum Network Explorer | South Holland |
| Quantum.Amsterdam | North Holland |
| QuantWare | |
| Quanza Engineering | North Holland |
| Quaras Software | North Holland |
| Quartess | Gelderland |
| QUBE ICT Solutions B.V. | North Holland |
| Quentic | |
| Quicargo B.V. | |
| Quint | North Holland |
| Quintor | Utrecht |
| Quipment | Gelderland |
| Quirem Medical B.V. | |
| QuiX Quantum B.V. | |
| QurieGen | |
| Qurin Diagnostics | |
| QuTech | South Holland |
| Qware ICT | |
| Qweb Internet Services B.V. | South Holland |
| Qwerty B.V. | North Brabant |
| Qwezz B.V. | Limburg |
| R&R Automatisering & Oplossingen | |
| R.E.D. Systemen B.V. | South Holland |
| R2 retail solutions B.V. | Limburg |
| Rabión Consultancy | South Holland |
| Racer | North Holland |
| Radboud Universiteit | Gelderland |
| Radboud Universiteit - Institute for Computing and Information Sciences | Gelderland |
| Radboud Universiteit - Mathematics | Gelderland |
| Radboud Universiteit Nijmegen - Faculteit der Natuurwetenschappen, Wiskunde en Informatica | Gelderland |
| Radboud University - Institute for Computing and Information Sciences | Gelderland |
| Radical Graphics Studios v.o.f. | |
| Radio Zeeland DMP | Zeeland |
| RAID Recovery Service | North Brabant |
| Rainbow Solutions B.V. | Gelderland |
| Raito B.V. | |
| Ralf van Veen | |
| RAM Infotechnology | Utrecht |
| RAM Mobile Data B.V. | Utrecht |
| RAMLAB | |
| Randstad Enterprise | |
| Randstad N.V. | North Holland |
| Ranj | South Holland |
| Rapid Photonics B.V. | |
| Rapidemic | |
| Raster | Gelderland |
| Rathenau Instituut | |
| Raymond van Bommel | |
| rb2 | |
| RDM Makerspace | |