| Eye4Software B.V. | Overijssel |
| Eyesiu Medicines B.V. | |
| F-ACT IT | Groningen |
| F.Institute B.V. | |
| Faber Electronics B.V. | Gelderland |
| FabFilter | North Holland |
| Facilitor | Overijssel |
| FacilityApps | North Holland |
| Factris Holding B.V. | |
| Fagron | South Holland |
| FairCode B.V. | |
| Fairphone | North Holland |
| Falcon Media | |
| Famas FM Solutions B.V. | North Brabant |
| Fameus Automatisering B.V. | Gelderland |
| Fantazm | North Brabant |
| Faraday Digital Agency | North Holland |
| FareHarbor B.V. | |
| Faster Forward B.V. | North Brabant |
| Fastned | North Holland |
| FastNet Africa B.V. | |
| Fastware B.V. | North Brabant |
| FC-IT | North Holland |
| Fe/male Switch | |
| Fe/male Switch | |
| Fectar | North Brabant |
| Feenstra CC | |
| Féju ICT Groep | North Brabant |
| FEKA ICT B.V. | Utrecht |
| Fellow Digitals | |
| Fellowmind | Utrecht |
| Felyx Sharing B.V. | |
| Femto Engineering B.V. | South Holland |
| Fenestrae | South Holland |
| Fenêtre | South Holland |
| Fero Payment Science B.V. | |
| Fervent Digital | |
| Festiviter | |
| FiberCore Europe B.V. | |
| Fiberplane B.V. | |
| Fibriant B.V. | |
| FieldBee | North Holland |
| Fiewallshop.nl | Utrecht |
| Figlo | South Holland |
| FikaWorks | |
| Filt.nl | |
| FIN2ITion | South Holland |
| Final Frontier | Flevoland |
| Finalwebsites | |
| Finch Capital | North Holland |