| Foss IT | Utrecht |
| Foster | Overijssel |
| Founda | North Holland |
| Foundation 4 Life | |
| Foundation Academy of Amsterdam | |
| Foundation for International Blockchain and Real Estate Expertise | South Holland |
| Founded in Groningen | Groningen |
| Four Digits | Gelderland |
| FourPoints Business Intelligence | North Holland |
| Fourthline B.V. | |
| Fourtress | North Brabant |
| Fox Games | |
| Fox-IT | South Holland |
| FOXXL Hosting B.V. | |
| Framer | |
| Framer B.V. | |
| Frank Techniek | |
| Fraudio | North Holland |
| Free Web Hosting Area.com | Zeeland |
| Freeday | |
| Freelance-IT | North Holland |
| Freenom | |
| Freepack Software B.V. | South Holland |
| Freez.it | Friesland |
| Freezing Hardware | Overijssel |
| Fresh Pixel | North Holland |
| Fresh Ventures Studio | |
| Fresh.li | South Holland |
| Freya Pharma Solutions B.V. | |
| Fring | |
| FrisBEE | Zeeland |
| FRISS | Utrecht |
| Frits | |
| Frits B.V. | |
| Fronteers | North Holland |
| Frontrow | |
| Fruition Partners | South Holland |
| FruitPunch Ai B.V. | |
| Fruto | Utrecht |
| Fryske Akademy | |
| Fuga | |
| Full Finance Consultants | |
| Fullfact Solutions B.V. | North Brabant |
| Fundament All Media | Groningen |
| Funded.club B.V. | |
| Funxtion International B.V. | |
| Furix B.V. | Gelderland |
| Fuse Web B.V. | |
| Fusernet B.V. | Utrecht |
| Fusion Engineering | |