| Enline Internet Solutions | Limburg |
| Enpicom B.V. | |
| Enreach | Flevoland |
| Enrise | Utrecht |
| ENT Solutions | Gelderland |
| Enterprise Summit | |
| Enterprise Websolutions | |
| Entersystems ICT Services | Gelderland |
| Entopic | |
| Entsys | |
| Envision | South Holland |
| Enzyre B.V. | |
| EnzyTag | Limburg |
| Epicor Components B.V. | South Holland |
| Epicwebsite | |
| Epify B.V. | |
| EPR Partner | Gelderland |
| ePublisher | |
| EQUA Electronics B.V. | Limburg |
| Equalture | South Holland |
| Equi4 Software | Utrecht |
| Equinox Global Services B.V. | South Holland |
| Equinox Software & Services | Flevoland |
| Erasmus MC | |
| Erasmus Research Institute of Management | |
| Erasmus University - Rotterdam School of Management | South Holland |
| Erasmus University Rotterdam | |
| ERC Automatisering | North Holland |
| Ergo Direct | North Brabant |
| Ergo2Go | South Holland |
| Ermeco Digitale Communicatie | Limburg |
| Erniesoft | |
| Esacape Educatif | North Brabant |
| Escape Software | North Brabant |
| Escom | |
| ESCUDERO | North Brabant |
| ESDNow.com | North Holland |
| Eshgro B.V. | North Brabant |
| Esko-Graphics B.V. | |
| ESOMAR | |
| Espalier B.V. | North Brabant |
| Esperante Development | |
| EsperantoXL | Utrecht |
| Esprit ICT Group | North Brabant |
| Estec Software | Groningen |
| Estrategy B.V. | |
| Eternalsun Spire | |
| Ethernet.nl | South Holland |
| Ethicqual | |
| Etiya | North Holland |