| ETPA | |
| Eudonet Stb | Utrecht |
| Euforie Online | |
| Euretos | Utrecht |
| EuroCollege | |
| Eurodrug Laboratories B.V. | |
| Eurodrug Laboratories B.V. | |
| Eurofiber | Utrecht |
| Eurofiber Nederland B.V. | Utrecht |
| European Association for Biometrics | North Holland |
| European Hardware Association | |
| European Startups | |
| European Test Services B.V. | |
| Europort Business School | |
| euroSRS | Gelderland |
| EV Biotech | |
| EVBox | North Holland |
| Eveleens Automatisering | |
| EvenHosten | |
| eVentures Africa Fund B.V. | |
| EventX | South Holland |
| Everox | |
| Evers en de Gier | |
| Evertalent | |
| Everynet | South Holland |
| Everything4Office Software B.V. | South Holland |
| Evi Software B.V. | North Brabant |
| EVO Venture Partners | North Holland |
| Evologics | North Brabant |
| EW Webservices Amersfoort | |
| Exa-Omicron | Flevoland |
| Exact | South Holland |
| Except Integrated Sustainability B.V. | |
| Exedo | South Holland |
| ExeVir Bio B.V. | |
| EXIN | Utrecht |
| EXLRT | |
| Exor | North Holland |
| ExoRobotics | |
| Expansion | South Holland |
| Experience Data | |
| Expert Solar Systems | |
| Experthosting.nl | |
| Expice B.V. | North Holland |
| ExSpect | North Brabant |
| Extravar | South Holland |
| ExtreemTek Netherlands | |
| eXtremelyWEB | Gelderland |
| Exvo.com B.V. | South Holland |
| Eye Security | |