| Entagy | |
| EnteraBio Ltd | Jerusalem District |
| Entrepreneurship Forum Israel | |
| Entro | Tel Aviv District |
| Entry | Tel Aviv District |
| Environmental Sustainability Innovation Lab | |
| Envitech Ltd | Tel Aviv District |
| ePiccolo Engineering Ltd | |
| Epitomee Medical | Haifa District |
| ePlace | Tel Aviv District |
| EPS Tech | Central District |
| Equitech Financial Consulting Ltd | Tel Aviv District |
| ERB | Tel Aviv District |
| ERG Solutions | Tel Aviv District |
| ERM Electronic Systems Ltd | Central District |
| ERPdex | Tel Aviv District |
| ERPR | |
| Escales | Tel Aviv District |
| Eshkol-it | Tel Aviv District |
| Etecad File Manager | Jerusalem District |
| EuroTech Communication Ltd | Northern District |
| EVchip | Central District |
| Ever Co., Ltd | |
| EverAfter Ltd | Tel Aviv District |
| Everest Digital Marketing | |
| Everybuddy Ltd | Tel Aviv District |
| Evogene Ltd | Central District |
| EVolution BMS Ltd | Jerusalem District |
| EvolutionVIP | Tel Aviv District |
| Ewave Online | Tel Aviv District |
| eWaveMD Ltd | Central District |
| Ex Libris Ltd | Tel Aviv District |
| Excellence Web Marketing | Jerusalem District |
| Execute | Tel Aviv District |
| Exelera | |
| Exero Medical | |
| eXite | Tel Aviv District |
| Exitvalley | Tel Aviv District |
| Expo Tel Aviv | |
| Expo-Net Systems | Tel Aviv District |
| Extra Mind | |
| EyeControl | Tel Aviv District |
| eyefeelit | Tel Aviv District |
| EyeYon | Central District |
| Eyron Software Group | Tel Aviv District |
| EZShift | Tel Aviv District |
| F2 Capital | Tel Aviv District |
| Fairfly | Tel Aviv District |
| FatFish | Jerusalem District |
| Fayrix Software | Tel Aviv District |