| Duma Optronics Ltd | Haifa District |
| DustPhotonics Ltd | |
| Dvash Smart Solutions Ltd | Tel Aviv District |
| dWallet Labs Ltd | Tel Aviv District |
| DYN | |
| Dynamo | Central District |
| E&M Computing | Tel Aviv District |
| E.D.Soft | Tel Aviv District |
| E.S. Embedded Solutions 3000 Ltd | Tel Aviv District |
| Earnix | Tel Aviv District |
| Earth & Beyond Ventures | Tel Aviv District |
| EasySec Solutions | |
| Easysite | Tel Aviv District |
| ECOBILL | Central District |
| EcoMotion | Tel Aviv District |
| EcoSoft - Software Development Ltd | Tel Aviv District |
| EDA Integrity Solutions Ltd | |
| Edea | Tel Aviv District |
| Edity Therapeutics | |
| EDP Group | Tel Aviv District |
| EdTech Israel | Tel Aviv District |
| Educational Information Technologies Alliance Network | Haifa District |
| Edusoft Ltd | Central District |
| Efcom | Central District |
| eHealth Ventures | Tel Aviv District |
| EiA | Tel Aviv District |
| Eitan Medical | Central District |
| EK Design | Tel Aviv District |
| Elad Software | Tel Aviv District |
| Elbit Systems Ltd | |
| Elcam Medical | |
| Eldan Software Systems Ltd | Tel Aviv District |
| Eldrotec | |
| Electric Fuel | |
| Element Security | Tel Aviv District |
| Elementor | |
| Elevation | Tel Aviv District |
| EliatHub | Southern District |
| Elmag Technologies Ltd | Tel Aviv District |
| Elmo Motion Control Ltd | |
| Elron Electronic Industries Ltd | Tel Aviv District |
| Elron Ventures Ltd | |
| Elspec Ltd | |
| Embedded RT Ltd | |
| Emerge Ventures | Tel Aviv District |
| eMonetization Ltd | Tel Aviv District |
| Endoron Medical Ltd | Central District |
| Endospan | Tel Aviv District |
| Energy Re-Connect Ltd | Tel Aviv District |
| Enertec Systems Ltd | Northern District |