| Spiria Digital, Inc. | Ontario |
| Spirit Blockchain Capital, Inc. | |
| Spitfire Computers Ltd | British Columbia |
| Spittin' Image Software, Inc. | British Columbia |
| Splash Entertainment and Communications, Inc. | Ontario |
| Splash Media Group | British Columbia |
| Splashdot | British Columbia |
| SplashGrafix, Inc. | Quebec |
| SPLICE Software | Alberta |
| Splinetech JavaScript Debugger | Quebec |
| SplitMango | British Columbia |
| Splitt | Quebec |
| Splus Software | |
| Spokal | |
| Spongelab Interactive | Ontario |
| Spongelii, Inc. | |
| Sponsorium | Quebec |
| Spordle | Quebec |
| SPORE Productions | Ontario |
| Sporobole | |
| Sportlogiq | |
| Sports AI | |
| Sports IQ Analytics, Inc. | |
| Spot Solutions | British Columbia |
| Spotekk Systems | Nova Scotia |
| Spoutnik, Inc. | Quebec |
| SPP Marketing Services, Inc. | Ontario |
| SPR Design | Quebec |
| SpriggHR | |
| Spring Activator | |
| Spring Collective | |
| Spring Impact Capital | |
| Spring Loaded Technology | |
| Springboard Atlantic | |
| Springboard Atlantic, Inc. | |
| SpringBoard Data Management | |
| Springs | |
| Springtime Software, Inc. | |
| Sprite Computer Systems Ltd | British Columbia |
| Sprngpod | |
| Sproing Creative | British Columbia |
| Sprott Shaw College | |
| Sprout Fund | |
| Spruce Tree Media Ltd | |
| Spry Agency, Inc. | Ontario |
| SpryPoint Services, Inc. | |
| SpryPoint Solutions, Inc. | Prince Edward Island |
| Spunkyworld Internet Services | British Columbia |
| Spyder Technology | British Columbia |
| Spyderbaby Productions | British Columbia |