| Speakeasy Solutions, Inc. | British Columbia |
| Spearhead Games | Quebec |
| Special Needs Computer Solutions, Inc. | Ontario |
| Specialty Web Design | |
| Specific Biologics, Inc. | |
| Specific Instruments Ltd | British Columbia |
| Specops Software | Ontario |
| Specto Technologies, Inc. | Quebec |
| Spectra FX, Inc. | Ontario |
| Spectra Media | Prince Edward Island |
| SpectraCodeIT | |
| Spectral Medical, Inc. | |
| Spectranet Connections, Inc. | Ontario |
| Spectratone Graphics Ltd | Ontario |
| Spectron Electronics, Inc. | Ontario |
| Spectrum Global Communications | Ontario |
| Spectrum Networks, Inc. | |
| Spectrum Pharma | |
| Spectrum Telecom Group Ltd | Ontario |
| Spectrum Web Solutions | |
| Spedassist Imagination Plus, Inc. | Ontario |
| Speech Mobility, Inc. | Quebec |
| SpeedFI | |
| Speedi Mobile Website | Ontario |
| SpeedLine Solutions, Inc. | British Columbia |
| Speedy PCB & Electronics, Inc. | Ontario |
| Speedy Site | |
| Spektrum | Quebec |
| Spellbook | Ontario |
| Spence Software | British Columbia |
| Spendsafe Financial Corporation | |
| SpendTheBits, Inc. | |
| Spexi | |
| Spexi Geospatial, Inc. | |
| SPG Digital Solutions, Inc. | Ontario |
| Sphere | British Columbia |
| Sphere Research Corporation | British Columbia |
| Spherika | Quebec |
| SpiceLogic, Inc. | Ontario |
| Spiderwort | |
| Spin Master Ltd | Ontario |
| Spin Web Solutions | Ontario |
| SpinaFX Medical | |
| Spincaster Productions | British Columbia |
| Spindle Biotech, Inc. | |
| Spineor | |
| Spinzo Corporation | |
| Spira Data Corporation | Alberta |
| Spire Systems, Inc. | |
| Spiri Robotics | |