| Ecopackers | |
| Ecopia AI | |
| EcoSynthetix, Inc. | |
| EcoSysIP, Inc. | Quebec |
| Écosystème startup de la Capitale-Nationale | |
| Écotech Québec | Quebec |
| Ecotel | |
| Ecotime | |
| Ecotuned Automobile | Quebec |
| Ecreate Marketing | |
| Ecritel, Inc. | Quebec |
| ECS Global | Ontario |
| ECTO | |
| Ecxo | |
| ED Tech Solutions, Inc. | |
| EDAC, Inc. | Ontario |
| Edaforce, Inc. | Quebec |
| EDAY | Ontario |
| Eddyfi | Quebec |
| eDealer | Ontario |
| Eden Advertising | Ontario |
| Eden P Host | Ontario |
| Edesa Biotech, Inc. | |
| Edge Dynamics | |
| Edge Industry Review | |
| Edge Interactive Publishing, Inc. | Ontario |
| Edgecom Energy | |
| Edgenda | Quebec |
| Edgetech Computing, Inc. | Alberta |
| Edgeware Systems Corporation | Ontario |
| EDI Gateway | Quebec |
| Edigenous Technology, Inc. | Ontario |
| EDIS Computers | British Columbia |
| Edisoft, Inc. | |
| Edison Communications | Quebec |
| Edit Undo Design | Ontario |
| EditCNC | British Columbia |
| Ediwise | Ontario |
| Edkent Media | Ontario |
| EDLS.COM | Ontario |
| EDMA Marketing | Ontario |
| Edmonton Advisory Council on Startups | Alberta |
| Edmonton Community Networks | Alberta |
| Edmonton Digital Services | |
| Edmonton New Technology Society | |
| Edmonton Startup Week | Alberta |
| Edmonton Unlimited | Alberta |
| Edmonton's Innovation Ecosystem | |
| EdmontonHost | Alberta |
| Edmontonwebmaster | Alberta |