| Evoluno | |
| Evosys | Brussels-Capital |
| EVS Broadcast Equipment S.A. | Liège |
| Ewapps | |
| eXiin | |
| EXO Biologics | |
| Exothera S.A. | |
| Expert-IT | Walloon Brabant |
| Expertmedia | |
| eXponent B.V. | East Flanders |
| EyeD Pharma S.A. | |
| EZGenerator | East Flanders |
| eZoulou | Brussels-Capital |
| Factory Forty | |
| FamousGrey | |
| Fan Arena | |
| Farmad N.V. | East Flanders |
| Faromedia | West Flanders |
| Fast IT Service | Namur |
| Features Analytics | |
| Federation of European Data and Marketing | |
| Federation of Telecoms Engineers of the European Union | Brussels-Capital |
| FEMAG S.A. | Walloon Brabant |
| Ferranti Computer Systems N.V. | Antwerp |
| Fiberklaar | |
| finance&invest.brussels | Brussels-Capital |
| Financial Systems Solutions S.P.R.L. | Walloon Brabant |
| Finindus | |
| Firmware | |
| FITCE Belgium | Brussels-Capital |
| FKS | Limburg |
| FLAGIS | East Flanders |
| Flagstone | |
| Flanders Make | |
| Flash Électronique | Liège |
| Fleet Complete | East Flanders |
| Flexina S.R.L. | Liège |
| Flexmail N.V. | Limburg |
| FloConsult | Walloon Brabant |
| FlowTribe | |
| FLUIDDA N.V. | |
| Flutteris | |
| FLYX | |
| Fonds de la Recherche Scientifique | Brussels-Capital |
| Forganiser | |
| Formatech New Technologies S.A. | Brussels-Capital |
| Formica | |
| Formlets | |
| Formlets | |
| Fox Biosystems | |