| EasySolution | |
| EatLocal | |
| EBAN | Brussels-Capital |
| Ebusiness.de | Namur |
| eCade S.R.L. | |
| ECAM Bruxelles | |
| Echo Shop | Brussels-Capital |
| Echosoft S.P.R.L. | |
| Eclips Comm.V | Antwerp |
| Eclipse Foundation AISBL | Brussels-Capital |
| École IT | |
| Econocom | Flemish Brabant |
| Ecoom | |
| ECT B.V.B.A. | Antwerp |
| Edebex | |
| Edge.be | Antwerp |
| EDGX | |
| EDP Web Design | Flemish Brabant |
| EDPNET Belgium B.V. | East Flanders |
| EDS Truck & Trailer Consulting | East Flanders |
| EDT Engineering | Hainaut |
| Edwin2Win S.P.R.L.U. | Hainaut |
| eeNews Europe | Walloon Brabant |
| Efficy S.A./N.V. | Brussels-Capital |
| Effitrax | |
| Effortel | |
| EIT Digital | Brussels-Capital |
| EIT Food | |
| Elex Biotech | |
| Elia System Operator S.A./N.V. | Brussels-Capital |
| Elipse | Antwerp |
| Elium | Walloon Brabant |
| Ello Mobile | |
| ElmediX | |
| Elmos | Antwerp |
| ELSI Solutions B.V.B.A. | |
| Elsyca N.V. | Flemish Brabant |
| Elyps S.A. | |
| Emakina Group | Brussels-Capital |
| Emma Legal | Antwerp |
| Emmanuel Finkels | Walloon Brabant |
| Emweb | Flemish Brabant |
| Encima B.V. | Antwerp |
| Encon | |
| End-2-End S.P.R.L. | Liège |
| EnergyVision | |
| Enersee.ai | |
| Enetricity B.V.B.A. | West Flanders |
| Enfocus | East Flanders |
| Entelec Control Systems B.V.B.A. | Limburg |