| Spaceship | Singapore |
| SpaceTech Asia | Singapore |
| Spark Systems Pte Ltd | Singapore |
| Sparkstation Pte Ltd | Singapore |
| Spartan Group | |
| Spaze Ventures | Singapore |
| Spaze Ventures Pte Ltd | Singapore |
| Specialized Therapeutics Asia Pte Ltd | |
| Spectrum Conferencing | Singapore |
| Speedoc | Singapore |
| Spenmo | Singapore |
| SpeQtral Pte Ltd | Singapore |
| SPH Ventures | Singapore |
| Spherium | Singapore |
| Spiral Ventures Pte Ltd | Singapore |
| Spoon Creative Online | Singapore |
| Springworks Interactive Century | Singapore |
| SQREEM Technologies Pte Ltd | Singapore |
| STACS | Singapore |
| Standard Digital Pte Ltd | |
| Staple AI Pte Ltd | Singapore |
| StarBase Pte Ltd | |
| StarHub Ltd | Singapore |
| Startup OI Pte Ltd | Singapore |
| Startup SG | Singapore |
| Startup Weekend Singapore | Singapore |
| Startup-O | Singapore |
| StartupEast Ventures | Singapore |
| StartUpJobs Asia | Singapore |
| StartupX | |
| StartupX Pte Ltd | Singapore |
| Starvision Information Technology Pte Ltd | Singapore |
| StashAway | Singapore |
| Stelexis Therapeutics | |
| StepVR Pte Ltd | Singapore |
| Stone Forest IT | Singapore |
| Storage Tech Pte Ltd | Singapore |
| Straits Knowledge | Singapore |
| StratAgile | |
| Strawberry Solutions | Singapore |
| Strontium Technology Pte Ltd | Singapore |
| STTelemedia | |
| SubQuery | Singapore |
| Subraa Singapore Pte Ltd | |
| Subscan | |
| SuiteLyfe Technologies Pte Ltd | Singapore |
| Sumica Pte Ltd | Singapore |
| Suneast Pte Ltd | Singapore |
| Sunpro | Singapore |
| Sunway Digital Pte Ltd | Singapore |