| Email Hippo Ltd | |
| Email Ladder | East Sussex |
| Email Marketing | Devon |
| EmailOctopus | |
| emap Publishing Ltd | |
| Embargo Lifestyle Ltd | |
| Embargo Lifestyle Ltd | |
| Embeddable | |
| Embedded Antenna Design Ltd | Buckinghamshire |
| Ember | |
| Ember Technology Ltd | |
| Ember Web | |
| Embryo | |
| Emerald ICT | West Yorkshire |
| Emerald Soft | |
| EmeraldPay Ltd | |
| Emerge Education | |
| Emergex Vaccines | |
| Emerging Europe | |
| Emerj | |
| Emirates NBD London Branch | |
| Emitwise | |
| Emneth Design | Cambridgeshire |
| Emobix Ltd | |
| EMOS Infineer Ltd | Ards and North Down |
| EMOS Information Systems Ltd | Hampshire |
| Empact Ventures | |
| Emperor | |
| EmpiricAI | |
| Empowerment Quality Engineering Ltd | Fermanagh and Omagh |
| Empresa Ltd | Norfolk |
| Empresaria Group plc | West Sussex |
| Emstopa | Kent |
| Emsworth Online | |
| Emteq Ltd | |
| Emu Analytics Ltd | |
| EMV Capital Ltd | |
| Enable Software | Warwickshire |
| Enable Technology | Shropshire |
| EnableIT Technologies Ltd | |
| enablesIT | Surrey |
| Enapps ERP | |
| EnAppSys Ltd | |
| Enara Bio Ltd | |
| Enate Ltd | |
| ENBIO Ltd | |
| Enbros | |
| Encelo Laboratories | |
| Enchanting Marketing Ltd | |
| enCircle | |