| Leadspicker | |
| LEC, s.r.o. | Prague |
| Lednet, s.r.o. | |
| Legrand, s.r.o. | Prague |
| LEITNER Slovensko, s.r.o. | |
| Lemonero | |
| Ler digital studio, s.r.o. | |
| Levebee, s.r.o. | |
| Level, s.r.o. | |
| LICA CZECH, s.r.o. | Central Bohemian |
| Lighthouse Ventures | |
| Limasoft, s.r.o. | |
| Limestone Digital | |
| Linelab.cz | |
| Linhart Marketing | South Moravian |
| LinuxBox.cz | Moravian-Silesian |
| LinuxEXPRES | South Moravian |
| Lira IS, s.r.o. | Prague |
| LitFin Capital, a.s. | |
| Livesport s.r.o. | Prague |
| Livesport, s.r.o. | |
| Liware, s.r.o. | |
| LK-Hardware, s.r.o. | |
| LLP Group | Prague |
| Localazy | |
| LocalDisti, s.r.o. | |
| Locus Workspace, s.r.o. | |
| Logio | |
| Look AI Ventures SICAV, a.s. | |
| LucidCircus | |
| Luigi's Box, s.r.o. | |
| Lukáš Barták | Prague |
| Lukáš Foldina | Moravian-Silesian |
| Luky's Soft | Prague |
| Lunaria, s.r.o. | |
| Lundegaard Slovensko s. r. o. | Prague |
| Lupa.cz | Prague |
| LutherOne plc | |
| M-Soft, spol. s r.o. | |
| M.C.TRITON, spol. s r.o. | |
| Machine Learning Prague | Prague |
| Macom Security, spol. s r.o. | |
| MacroToolworks.com | Hradec Králové |
| MACROWARE, s.r.o. | South Moravian |
| Mader.cz | Liberec |
| Madesense.digital, s.r.o. | Prague |
| MADFINGER Games, a.s. | |
| MADFINGER Games, a.s. | |
| Magmio, a.s. | |
| MAIT | |