| VMB Automation | North Brabant |
| VMV cad-adviseurs B.V. | South Holland |
| VobeSoft | North Brabant |
| Vobis Computers | Limburg |
| VOC Capital Partners | North Holland |
| Voclarion | North Holland |
| Vodafone Libertel B.V. | Limburg |
| VoiceTrading | North Holland |
| VolkerWessels Telecom | Utrecht |
| Voltec CV | Limburg |
| VONQ | South Holland |
| Voormedia | North Holland |
| Vorm IT | North Holland |
| Vortex Capital Partners | North Holland |
| Vosbor Exchange B.V. | |
| VOSviewer | |
| Voyc | North Holland |
| Voys B.V. | Groningen |
| VR-Innovations | |
| VR-Software | |
| VRHTI | North Holland |
| Vrije Hogeschool | |
| Vrije Universiteit Amsterdam | North Holland |
| Vrije Universiteit Amsterdam - Computer Science | North Holland |
| Vrije Universiteit Amsterdam - Department of Mathematics | North Holland |
| VRTech Group | |
| VSH Tech | |
| VSN Systemen B.V. | Limburg |
| VSPARTICLE | South Holland |
| VSTEP B.V. | |
| VSTEP B.V. | |
| VTTI | |
| VTU Telecom | North Holland |
| VUE.JS Amsterdam | North Holland |
| VV-Software | North Brabant |
| VWA Digital Agency | Flevoland |
| VX Company | Utrecht |
| W&S Belenux B.V. | North Brabant |
| W&W Quality Software B.V. | Flevoland |
| Waag Technology and Society | North Holland |
| Waalstad Internet | Gelderland |
| Waalweb | North Holland |
| Waarde Capital | North Holland |
| Wageningen University & Research | Gelderland |
| Wageningen University & Research on Social Media - Bioinformatics | Gelderland |
| Wakoopa | North Holland |
| WalMar | Drenthe |
| Wambay Software B.V. | |
| Wanbound | Utrecht |
| Wardenburg Beveiliging & Telecom | Overijssel |