| UP learning | Gelderland |
| Up!timize B.V. | |
| Up2technology | |
| UpLeveled B.V. | |
| Uprise | Overijssel |
| Upscene Productions | North Brabant |
| UpToMore B.V. | |
| Uptrends B.V. | South Holland |
| Urban Mobility Systems | |
| Urbandine B.V. | |
| Urbee | Utrecht |
| UReason | |
| URLfilterDB B.V. | Gelderland |
| Usabilityweb.nl | North Holland |
| Usabilla B.V. | North Holland |
| Uselab | North Holland |
| Usenet4u B.V. | Limburg |
| Userguest | North Holland |
| USoft B.V. | North Holland |
| Utilis | Flevoland |
| Utomik B.V. | North Brabant |
| Utrecht Holdings | |
| Utrecht University - Department of Information and Computing Sciences | Utrecht |
| UtrechtInc | |
| V.S.M. Automatisering B.V. | North Brabant |
| V4-Automatisering | Gelderland |
| VAA Data Works | North Brabant |
| Vabi | South Holland |
| Vactik | Utrecht |
| Valadis B.V. | Overijssel |
| Valk Solutions | Utrecht |
| Vallei-ICT | Gelderland |
| VallueBlue | Utrecht |
| ValueGiG | North Brabant |
| Valyuu B.V. | South Holland |
| Van Beek Elektrotechniek B.V. | Utrecht |
| Van den Broek Systemen B.V. | North Brabant |
| Van Dulst Automatisering | North Brabant |
| Van Eldijk | Utrecht |
| van Gelder Systems | |
| Van Gils Computersystemen | North Brabant |
| Van Lanschot Kempen N.V. | |
| Van Room Automatisering | Flevoland |
| Van Tuinen Webdesign | Drenthe |
| van Zon Internet | North Brabant |
| Vandebron Energie B.V. | |
| Vanderlande Industries B.V. | |
| VanDerLee | North Holland |
| Vanguard Entertainment Group | North Holland |
| VanMoof B.V. | North Holland |