| Viadesk B.V. | North Holland |
| Vialis B.V. | Utrecht |
| VIB Netwerken | Groningen |
| Vibe Group | North Holland |
| Vibes Consultancy | North Brabant |
| ViCi Consulting | North Brabant |
| ViciniVax | Groningen |
| Vico Therapeutics | |
| Vict Informatici | North Brabant |
| Vicus eBusiness Solutions B.V. | Utrecht |
| Videtur B.V. | North Brabant |
| Vidicode | South Holland |
| Viewbook B.V. | |
| Viewport.ai | |
| Viggo Smart Hotel | North Holland |
| Vigor & Zest | |
| Vijfhart | Gelderland |
| VIKTOR | |
| Villari B.V. | |
| ViNotion B.V. | |
| VIP Internet Provider B.V. | Gelderland |
| Viqal | North Holland |
| Vir2biz | Gelderland |
| Viritel B.V. | North Holland |
| ViroNovative B.V. | |
| Virtual IT Dude | |
| Virtual Reality Learning Lab | |
| VirtualMedSchool | South Holland |
| Visionit Systems B.V. | |
| VisionLabs B.V. | North Holland |
| Visionplanner B.V. | Utrecht |
| Visma Circle B.V. | North Brabant |
| Visma Roxit | Gelderland |
| VisserTek | Limburg |
| Vistaprint Netherlands B.V. | |
| VistaWeb | Zeeland |
| Visual Steps B.V. | North Holland |
| Visual Systems | Flevoland |
| Visualisatie.net | Drenthe |
| VISUfarma B.V. | |
| Visymo Universal Search Group | |
| VitalFluid B.V. | |
| Vitestro | |
| VitroScan | |
| VitroScan B.V. | |
| VIVA Veterinary B.V. | Overijssel |
| Vive Invest B.V. | |
| VLAG Graduate School | |
| Vlasveld Computers | South Holland |
| VLC & Partners | |