| Libertatis Ergo Holding B.V. | |
| Libra Energy B.V. | |
| Libra Service Automatisering B.V. | North Brabant |
| LibRT B.V. | North Holland |
| Liemar Software B.V. | North Brabant |
| LIFT Software | North Holland |
| LiGalli | |
| Lightbase | |
| Lightning Video Editors | |
| Lightyear | |
| Lime Networks B.V. | |
| Lindeman B.V. | North Holland |
| Linden-IT | North Holland |
| Lines broadcast B.V. | Gelderland |
| Lingewoud | North Brabant |
| Lingua Franca Consulting B.V. | |
| linkbuildingking.nl | South Holland |
| Linker IT Software B.V. | North Holland |
| Linkit B.V. | Utrecht |
| Linkody | North Holland |
| LinXis B.V. | |
| LioniX B.V. | Overijssel |
| LionVolt B.V. | |
| Lipid Knowledge B.V. | |
| Liquit | South Holland |
| LiteVax B.V. | |
| Little Chicken Game Company | |
| Little Robots | |
| Little Rocket | Overijssel |
| Livecom | Utrecht |
| LivingIT | |
| Lizard Global | South Holland |
| LLBLGen Pro | South Holland |
| Local Heroes Worldwide B.V. | |
| LocalFocus | |
| Localhosting | Limburg |
| Localie B.V. | |
| Locamation B.V. | Overijssel |
| Lodder Software | South Holland |
| Logaholic Web Analytics B.V. | North Holland |
| Logi Label | South Holland |
| Logic Vision | South Holland |
| LogicTrade | Overijssel |
| LoginPC | North Holland |
| LogiVert | North Brabant |
| LogSentinel | |
| Loop Robots | South Holland |
| LoopingOne | North Holland |
| Loqus | South Holland |
| LowBudgetPC | Zeeland |