| LaptopCentrale B.V. | Friesland |
| Lardenoije informatisering | Limburg |
| Laska Computers | North Holland |
| LassooyDesign | North Holland |
| Last Mile Solutions | South Holland |
| Laton Ventures | |
| Laumans Techniek B.V. | Limburg |
| Launchdesk | |
| Launchlab | Overijssel |
| LAVA Therapeutics N.V. | |
| LCG B.V. | |
| Lead Pharma | |
| LEAD Solutions B.V. | Utrecht |
| Leadbouwers | |
| LeadBoxer B.V. | |
| Leadgate Europe | South Holland |
| Leadinfo | South Holland |
| Leadrs | North Brabant |
| Lean Startup Workshop | Limburg |
| Leap24 B.V. | |
| Leapfunder | North Holland |
| Leapmotor International B.V. | |
| Leapp | |
| Learned | |
| LearningTrain | North Holland |
| Learnit.nl | North Holland |
| Leaseweb | |
| Leeman Automatisering | Utrecht |
| LegalThings | Utrecht |
| Lege Cartridge NL | South Holland |
| Leiden Bio Science Park | South Holland |
| Lemon Solutions | |
| Lemontree | South Holland |
| Lenneke Meijer | South Holland |
| LENS Business Software B.V. | North Holland |
| Lepaya | North Holland |
| Leppink Automatisering & Computers | Overijssel |
| LeQuest B.V. | |
| LetsGoDigital | |
| LetsStat | Friesland |
| Level Level | |
| Level2 Ventures B.V. | |
| Levenue | North Brabant |
| Levi9 | North Holland |
| Lex IT | South Holland |
| Leyden Laboratories B.V. | |
| LeydenJar | North Brabant |
| Liad Electronics Breda B.V. | North Brabant |
| Liander N.V. | |
| Libelnet | Limburg |