| LayerX | Tel Aviv District |
| Leadcom Integrated Solutions Ltd | Central District |
| Leaders | |
| Learn-C.org | |
| learn-cpp.org | |
| learn-html.org | |
| learn-js.org | |
| LearnCS.org | |
| LearnJavaOnline.org | |
| LearnnPython.org | |
| LearnPython.org | |
| LearnRubyOnline.org | |
| Lehavot | |
| Leos Media & Interactive | |
| Let Lab | Northern District |
| Letstok Technologies Ltd | |
| LeumiTech | Tel Aviv District |
| Liacom Systems Ltd | |
| Liav Fund | Tel Aviv District |
| Liberty Pixel Ltd | |
| Life Micheal | Tel Aviv District |
| Lightricks Ltd | Jerusalem District |
| LightSolver | |
| Limaca Medical | Northern District |
| Linnovate | Tel Aviv District |
| Linnovate Technologies Ltd | Tel Aviv District |
| LionBird (Ventures) Ltd | Tel Aviv District |
| LipoCure | Jerusalem District |
| Lishtot | Jerusalem District |
| Litigate | Tel Aviv District |
| Livecity | Tel Aviv District |
| Livecycle, Inc. | |
| LiveDNS | Central District |
| LiveShop | Tel Aviv District |
| Locusview | Tel Aviv District |
| Log-On | Tel Aviv District |
| Logate | Tel Aviv District |
| LogicPC | Tel Aviv District |
| Logtel Computer Communications Ltd | Central District |
| lool Ventures | Tel Aviv District |
| Loops | Tel Aviv District |
| Loora Ltd | |
| LTU | |
| Lumenis Be Ltd | Northern District |
| Lutris Pharma | |
| Lydus Medical | Central District |
| Lynx | Tel Aviv District |
| Lynx | |
| M.G.R (Zahor) Technologies Ltd | Central District |
| Maabarot Group | |