| Itzhaki-Weinberger Consultants Ltd | Tel Aviv District |
| IVC Research Center | Tel Aviv District |
| Ivory | Tel Aviv District |
| J-Town Internet Services Ltd | Jerusalem District |
| JAL Ventures Ltd | Tel Aviv District |
| JaxBio | Central District |
| JBS | Tel Aviv District |
| jbStore | Tel Aviv District |
| JCOM | |
| Jerusalem College of Technology | Jerusalem District |
| Jerusalem Global Ventures | Jerusalem District |
| Jerusalem Hub | Jerusalem District |
| Jerusalem Industrial Areas | Jerusalem District |
| Jerusalem Multidisciplinary College | |
| Jerusalem Perl Mongers | Jerusalem District |
| Jerusalem Sky Software Solutions | Jerusalem District |
| Jerusalem Style | Jerusalem District |
| Jerusalem University College | Jerusalem District |
| Jerusalem Venture Partners | Jerusalem District |
| JEST | Jerusalem District |
| JetServer Web Hosting | Tel Aviv District |
| JFrog Ltd | Central District |
| JLM-BioCity | |
| JNext | Jerusalem District |
| Jobnet Online Ltd | |
| John Bryce | |
| Join Digital Talent Agency | Tel Aviv District |
| Joinweb Ltd | Tel Aviv District |
| Jolt | |
| Joomla | Tel Aviv District |
| Jordan Valley Semiconductors Ltd | Northern District |
| Joy Ventures | Tel Aviv District |
| JSCapital | Tel Aviv District |
| Jumpspeed | |
| Jumpspeed Ventures | Jerusalem District |
| Justt Ltd | Tel Aviv District |
| K Logic | Tel Aviv District |
| K&E Design | Tel Aviv District |
| K.O.S High-Tech Outsourcing Solutions Ltd | Central District |
| K2View | Northern District |
| Kadimastem Ltd | Central District |
| Kaedan Capital Ltd | Tel Aviv District |
| Kahena | |
| KAHR | Central District |
| Kahun Ltd | |
| Kahuna Digital | Tel Aviv District |
| Kala | Central District |
| Kamari Pharma | |
| KamaTech | Tel Aviv District |
| Kamatech Accelerator | Tel Aviv District |