| 270net Technologies, Inc. | Maryland |
| 27labs | California |
| 28 Media | |
| 280Bio, Inc. | |
| 28Bio | |
| 28msec, Inc. | California |
| 2:45Tech, LLC | Tennessee |
| 2AB, Inc. | Alabama |
| 2am.tech | Florida |
| 2b Interactive | Tennessee |
| 2B Unique Web Solutions | |
| 2b1, Inc. | California |
| 2btechinc | |
| 2C Development Group | Kansas |
| 2Checkout | Florida |
| 2connex | Massachusetts |
| 2CoolTek, LLC | Minnesota |
| 2D Imaging, Inc. | California |
| 2D Technology Group, Inc. | Maryland |
| 2d3D | Florida |
| 2DA Analytics, Inc. | Texas |
| 2DaMax Marketing, LLC | |
| 2DEngine | New York |
| 2Dudes Digital Design | |
| 2GBHosting | North Carolina |
| 2J2M, Inc. | |
| 2K Medical Billing Systems | California |
| 2K Software Solutions, Inc. | |
| 2K Software, LLC | |
| 2Leaf | |
| 2Morrow, Inc. | Washington |
| 2msolutions, Inc. | Texas |
| 2muchcoffee | |
| 2nd Address | California |
| 2nd Line Marketing | |
| 2nd Watch, Inc. | Washington |
| 2nd.MD | Texas |
| 2ndNature Software, Inc. | California |
| 2ndWave Software | Arizona |
| 2Novas | |
| 2pensmedia | Illinois |
| 2Plus2 Partners, Inc. | California |
| 2POINT, Inc. | |
| 2Rivers Media | |
| 2ROCK1 Media, LLC | |
| 2S Marketing, LLC | Nevada |
| 2SD Toxicology Services, LLC | North Carolina |
| 2Secure.biz | New York |
| 2see Technology, LLC | Pennsylvania |
| 2seventy Bio, Inc. | Massachusetts |