| 1st Click Consulting | Colorado |
| 1st Design | California |
| 1st Free Solitaire | Virginia |
| 1st National | Michigan |
| 1st Playable Productions, LLC | New York |
| 1st Provider's Choice | |
| 1st Source Web | California |
| 1st Televoice, Inc. | California |
| 1stChoice Web Host | New York |
| 1STCONS, LLC | Wyoming |
| 1stFlash | New York |
| 1stHostWeb.com | Texas |
| 1stVoice | California |
| 1TARGET | |
| 1three Consulting | |
| 1to1Network, Inc. | |
| 1uhost.com | Florida |
| 1UP SEO | |
| 1upcomputer | Virginia |
| 1upHealth | Massachusetts |
| 1Webshop | Georgia |
| 1World Online | California |
| 1WorldSync | Illinois |
| 1×1 Impression SEO, LLC | |
| 1→10, Inc. | |
| 2 Fish Company, LLC | Michigan |
| 2 Guys Design | |
| 2 Levels Above, LLC | Texas |
| 2 the Top Web Design & Marketing | Tennessee |
| 2-U Technology, LLC | New Hampshire |
| 2-Way Communications Service, Inc. | New Hampshire |
| 20 Miles North Web Design | |
| 20-20 Business Solutions, Inc. | New Jersey |
| 20/20 BioLabs | |
| 20/20 Capital Group | Pennsylvania |
| 20/20 Gene Systems | Maryland |
| 20/20 Onsite | |
| 20/20 Software, Inc. | Connecticut |
| 2000Charge, Inc. | California |
| 2000domain.net | Pennsylvania |
| 201 Creative, LLC | |
| 202 Partners, LLC | Maryland |
| 2020 Vision Digital | |
| 2020-Innovation, Inc. | Florida |
| 2029 Web Design, LLC | |
| 2044 Media, LLC | |
| 206 Ortho | Massachusetts |
| 2060 Digital | |
| 20Fathoms | Michigan |
| 20free Web Pages | California |